Measuring DNA Damage and Repair in Mouse Splenocytes

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FEATURING 100% PURE ITALIAN DESIGN AND ENGINEERING. Passionate Innovation. Over thirty years of continuous research. gamma-H2AX Recombinant Monoclonal Antibody [BLR053F] Reactivity Human, Mouse. Applications WB, IP, IHC, ICC, IHC-IF. Immunogen surrounding Serine 140.

Gamma h2ax antibody

2021-04-10 · Flow Cytometry: gamma H2AX [p Ser139] Antibody [NB100-384] - Analysis of gamma-H2AX in Etoposide Treated Jurkat Cells. Cells were treated for 3 hrs in 5ug/ml etoposide, fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 million cells were stained with 0.5 ug anti-KLH or anti-H2AX NB100-384 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). FITC anti-H2A.X Phospho (Ser139) Antibody - H2A.X is a 14 kD basal histone and a member of the H2 histone family. Histone H2AX: Products.

Produced in Rabbit. Catalog No. Size. A300-081A 0.1 ml.

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Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody, Histone Gamma-H2A.X (Phospho S139) Clone RM224 quantity Add to cart SKU: 31-1104-00 Categories: Histone H2A.Z Acetylation , Histone Modification , Primary Antibodies , Rabbit Monoclonal Tags: 31-1104-00 , 31-1104-00-S , 31110400 , 31110400S Rabbit Polyclonal gamma H2AX Antibody Provider product page Novus Biologicals - NB100-384 Antibody type Polyclonal Description Immunogen affinity purified. The epitope maps to a region surrounding phosphorylated serine 139 of human histone H2AX. Reactivity Human, Mouse, Rat, Canine Host Rabbit Isotype IgG Vial size 0.1 ml Concentration 1.0 mg Balajee, A. S. & Geard, C. R. Replication protein A and gamma-H2AX foci assembly is triggered by cellular response to DNA double-strand breaks. Exp Cell Res 300, 320–334 (2004).

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Histone H2A.X is a variant histone that represents approximately 10% of the total H2A histone proteins in normal human fibroblasts (1). H2A.X is required for checkpoint-mediated cell cycle arrest and DNA repair following double-stranded DNA breaks (1). Phospho-H2AX or γ-H2AX- is a marker of DNA double-stranded breaks and can therefore be used to monitor DNA repair after, for example, irradiation.

Cells were treated for 3 hrs in 5ug/ml etoposide, fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 million cells were stained with 0.5 ug anti-KLH or anti-H2AX NB100-384 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Gamma‐H2AX is used widely as DNA damage marker in vitro, but its use for genotoxicity assessment in vivo has not been extensively investigated. Here, we developed an image analysis system for the precise quantification of the gamma‐H2AX signal, which we used to monitor DNA damage in animals treated with known genotoxicants (EMS, ENU and doxorubicin). Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 Anti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. Compare gamma H2AX [p Ser139] Antibody (3F2) NB100-74435 from Novus Biologicals on Biocompare.com FITC anti-H2A.X Phospho (Ser139) Antibody - H2A.X is a 14 kD basal histone and a member of the H2 histone family.
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Radiat an immunocytochemical assay with antibodies recognizing gamma-H2AX has Since no freeware programme for the analysis of gamma-H2AX foci exists for a Gamma-H2AX foci; Ionising radiation; Fluorescence microscopy; Automated Production of an Avidin Conjugated Antibody Pretargeting Agent Optimising a method for visualising gammaH2AX-foci after irradiation with Eftersom överflöd av γ H2AX indikerar omfattningen av DSB, identifierade vi först (anti- γ H2AX antibody or anti-53BP1 antibody or anti-BCLAF1 antibody for 4-Hydroxysmörsyra, GHB, gamma-Hydroxybutyrat Anti-VGPC, Anti-VGKCs, Voltage-gated potassium channels antibody, LGI-1, CASPR2 Gamma- H2AX. of specific immuno- globulin E antibodies relevant in food allergy to crustaceans and Long time persistence of residual 53BP1/gamma-H2AX foci in human Effekt av oral administrering av metylenblå MMX-tabletter på dubbelsträngad DNA-skada bedömd genom GammaH2AX-analys av kolonbiopsiprover testis for both 111-InCl3 and 111-In attached to an antibody, called Rituximab, Our results also indicate that the use of the γ-H2AX biomarker can be useful (författare); Quantitative γ-H2AX immunofluorescence method for DNA double-strand break analysis in testis and liver after intravenous administration of Background: The Bruton's Tyrosine Kinase (BTK)‑inhibitor ibrutinib is highly active in mantle cell lymphoma (MCL) but may inhibit response to anti‑CD20 antibody behandlings-inducerad γ-H2AX foci i arm 1 med 1000, Bethyl Laboratories) och γ-H2AX Term Safety of Nivolumab (Anti-Programmed Death 1 Antibody,. 53BP1 and γ-H2AX proteins that produce distinct foci co- delvis 2 timmar efter γ-bestrålningen Pennsylvania, USA, for donation of antibodies to 53BP1;. rerna γ-H2AX och 53BP1 visade vi att strålbehandling efter neo- adjuvant orescence staining with Ku70 mouse-monoclonal antibody.

UniProt Code History. P16104, Q4ZGJ7, Q6IAS5. Gene Synonyms. H2AX, H2a.x, H2afx, H2A/X.
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Antigen, Human. A gamma-H2AX kit and antibody allows the assessment of DNA damage and DNA repair for ELISA based assays, immunohistochemistry or flow cytometry. The S139 phosphorylated H2AX, termed gamma -H2AX, marks the site of DNA double-strand breaks and serves to recruit cell cycle checkpoint and DNA repair 5 Mar 2021 Phosphorylated on Ser-140 (to form gamma-H2AX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). 28 Jun 2010 In mammalian cells, γ-H2AX is formed when H2AX is ished binding of anti-γ- H2AX antibody to Y142 mutants, as this antibody was developed After removal from incubation, cells were kept at room temperature before extraction.

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H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). Anti-Gamma H2AX (phospho-Ser139) antibody, Rabbit monoclonal. 1 Product Result. | Match Criteria: Product Name, Property. Synonym: H2AX Ser139 p, Histone H2AX (phospho ser139) Product #. Clonality. I use MCF-7 cell, treated with 0.5uM Doxorubicin 24 hours, did WB, and used two different gamma H2AX antibodies to detect ( #2577 in Cell signaling, rabbits and #05-636 in Millipore, mouse) I View application images and datasheets for 754 anti gamma-H2AX Antibody antibodies from 38 leading antibody suppliers, plus reviews and the top related antibodies Anti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. This purified mAb is highly specific for phospho-Histone H2A.X (Ser139) 2016-03-31 · Anti-gamma H2A.X (phospho S139) antibody [EP854 (2)Y] (ab81299) Research with confidence – consistent and reproducible results with every batch.

- Find MSDS or SDS, a COA, data sheets and more information. FITC anti-H2A.X Phospho (Ser139) Antibody H2A.x, H2a/x, Histone 2A, Histone 2A.X, Gamma-H2AX Isotype Mouse IgG1, κ Ave. Rating Submit a Review Product Citations publications. HeLa cells untreated (open histogram) or treated with Nocodazole for 24 hours gamma H2AX Antibody - read details of Novus Biologicals, LLC antibodies in the SelectScience.net Antibody products and suppliers directory Rabbit Polyclonal gamma H2AX Antibody Provider product page Novus Biologicals - NB100-384 Antibody type Polyclonal Description Immunogen affinity purified.